Mickey Mouse embarks on a vacation from Burbank to Pomona, sneaking his loyal canine companion Pluto onto the train by hiding him in his suitcase. The conductor, Pete, relentlessly pursues Mickey and Pluto as they attempt to evade detection, leading to a series of hilarious misadventures through various parts of the train. In the end, Mickey and Pluto successfully evade capture and arrive at their destination, celebrating with a joyful reunion.